Mosimane has kept the core of the available players who did duty at the 2026 World Cup, but has added some new names to the list.
One of those is in-form Chicago Fire winger Puso Dithejane, who has been in scintillating form in Major League Soccer.
Mamelodi Sundowns striker Cassius Mailula is another back in contention, while Cyprus-based Luther Singh gets the nod.
With the injured Evidence Makgopa unavailable, his Orlando Pirates teammate Yanela Mbuthuma is on the list.
The vastly experienced Themba Zwane is also still in contention after his horror World Cup, while at the other end of the spectrum, Pirates youngster Cemran Dansin is on the list.
Olwethu Makhanya and Ime Okon have both been selected with Mbekezeli Mbokazi injured, while there is a potential recall for Sundowns left-back Fawaaz Basadien.
Bafana play Guinea at the Royal Bafokeng Sports Palace on September 26, and Eritrea in neutral Cairo four days later. They will then face Egypt in a friendly at the same venue.
Bafana Bafana squad:
Goalkeepers: Ronwen Williams (Mamelodi Sundowns), Sipho Chaine (Orlando Pirates), Ricardo Goss (Mamelodi Sundowns)
Defenders: Aubrey Modiba (Mamelodi Sundowns), Samukele Kabini (Molde FK), Fawaaz Basadien (Mamelodi Sundowns), Kamogelo Sebelebele (Orlando Pirates), Thapelo Morena (Mamelodi Sundowns), Thabang Matuludi (Polokwane City), Olwethu Makhanya (Rangers), Khululani Ndamane (Mamelodi Sundowns), Ime Okon (Hannover 96), Lebone Seema (Orlando Pirates), Nkosinathi Sibisi (Orlando Pirates), Tshepo Mashiloane (Sekhukhune United)
Midfielders: Teboho Mokoena (Mamelodi Sundowns), Lebohang Maboe (Kaizer Chiefs), Cemran Dansin (Orlando Pirates), Sphephelo Sithole (CD Tondela), Bathusi Aubaas (Mamelodi Sundowns), Brooklyn Poggenpoel (Durban City)
Forwards: Iqraam Rayners (Mamelodi Sundowns), Yanela Mbuthuma (Orlando Pirates), Lyle Foster (Houston Dynamo), Cassius Mailula (Mamelodi Sundowns), Oswin Appollis (Orlando Pirates), Puso Dithejane (Chicago Fire), Tashreeq Matthews (Mamelodi Sundowns), Relebohile Mofokeng (Royale Union Saint-Gilloise), Themba Zwane (Mamelodi Sundowns), Ronaldo Van Neel (Golden Arrows), Thapelo Maseko (FC Copenhagen), Tshepang Moremi (Orlando Pirates), Luther Singh (AEL Limassol), Shandre Campbell (KV Kortrijk)