Kim, who shot a level-par 71, selected a three iron from his bag and struck his ball to the front fringe where it rolled across the putting surface and dropped in the hole.
"I've had about six holes-in-one before but this is the most memorable," he said of his moment of magic on the 17th which had never witnessed a hole-in-one in previous Opens at Troon.
The previous longest hole-in-one at the Open was the 212-yard ace Frank Lickliter II made at the fifth hole at Royal Lytham & St Annes in 2001.
Kim was five over after three rounds.